EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex.
EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex.
Water hardness can be measured using a procedure known as complexometric titration by adding a known concentration of the chelating agent EDTA through a burette to a sample containing an unknown amount of calcium and magnesium ions. EDTA reacts and captures these metal ions creating a larger metal complex.
Methyl orange is a popular pH indicator that is used in the titration. The colour of the solution turns red when methyl orange is used as an indicator of acid. When methyl orange is mixed with a base, the result is yellow, or the colour changes to yellow. As a pH symbol, methyl orange is used.
Lead(II) nitrate is an inorganic compound with the chemical formula Pb(NO3)2. It commonly occurs as a colourless crystal or white powder and, unlike most other lead(II) salts, is soluble in water.
Ferrous Sulfate is a greenish, yellow-brown or white, odorless, crystalline (sand-like) powder. It is used in water treatment and in making other Iron compounds, and as a fertilizer and food additive.
Ferrous metals are hard, heavy, have high density, fragile, not malleable, ductile, magnetic, and rust. Non-ferrous metals are light, malleable, not magnetic, and more expensive. There are different ferrous metals, and each one has its uses. For example, steel is used in buildings, skyscrapers, and structures.
Chemically, the compound is represented by the formula C6H12O6 and could be found in plants and animals in huge quantities. Its common isomers include the L-glucose and D-glucose even though the fructose (i.e. which is the keto-form) is a widely established isomeric compound.
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