The purpose of adding sodium thiosulfate is to remove the excess iodine from the reaction mixture. This is important because iodine is a strong oxidizing agent and can cause side reactions with the organic compounds in the mixture.
EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex.
Buffer capsules are used for preparation of phosphate buffer solutions with stable pH, buffer capacity and low temperature coefficients. Buffer capsules are easy to use and dissolve easily. After that the pH value is automatically set at the necessary level.
Buffer solution, pH 4.0 ± 0.02 is used to establish and maintain an ion activity within narrow range. It is most commonly used to establish hydrogen-ion activity for the calibration of pH meters, in analytical procedures. It is also used to maintain stability of various dosage forms.
Nickel (II) Sulphate Heptahydrate-It is used in electroplating and as a chemical intermediate to produce other types of nickel compounds. Nickel Sulfate is a by-product of copper refining and it naturally occurs as a rare mineral which is retgersite.
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